The heartbroken singer sat next to his children during the service which attracted scores of well wishers.
Ann-Marie Elliot died three weeks ago in the United States of undisclosed complications. She was 32.
Nesbeth teared up last week while performing a tribute song to his deceased wife at a Vale Royal reception hosted by Prime Minister Andrew Holness. Nesbeth has the chart topping single My Dream currently taking the world by storm.